minimum essential medium mem α (Thermo Fisher)

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Thermo Fisher minimum essential medium mem α
Minimum Essential Medium Mem α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/minimum+essential+medium+mem+%CE%B1/SOB+medium/pm41369222-291-5-10
Average 99 stars, based on 1 article reviews

minimum essential medium mem α - by Bioz Stars, 2026-09

99/100 stars

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Cell Culture:

Article Title: C-type natriuretic peptide improves maternally aged oocytes quality by inhibiting excessive PINK1/Parkin-mediated mitophagy
Article Snippet: .. Only COCs with morphological integrity and a distinct germinal vesicle (GV) were cultured in basic culture medium consisted of Minimum Essential Medium (MEM)-α (Life Technologies, New York) supplemented with 3 mg/mL bovine serum albumin and 0.23 mM pyruvate at 37°C under an atmosphere of 5% CO2 in air with maximum humidity. ..

Article Title: Human alphacoronavirus replication and innate immune induction in airway culture systems.
Article Snippet: Human A549 cells expressing the receptor ACE2 (A549ACE2) (16, 39) were cultured in RPMI 1640 (Gibco catalog no. 11875) supplemented with 10% fetal bovine serum (FBS) and 1× penicillin-streptomycin. .. LLC-MK2 cells were cultured in Minimum Essential Medium (MEM) α (Gibco 12571063) supplemented with 10% FBS and 1× of penicillin-streptomy cin. .. MRC-5 (CCL-171) (77) and HT1080 (HT1080/CCL-121) (78) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco 11965) supplemented with 10% FBS, 1× of penicillin-streptomycin.

Article Title: GREM1 deficiency induced bone marrow adipose niche promotes B-cell acute lymphoblastic leukemia disease progression.
Article Snippet: .. The cells were cultured in Minimum Essential Medium (MEM) α (Thermo Fisher, Waltham, MA) with 10% fetal bovine serum (Thermo Fisher, Waltham, MA) at 37 C and 5% CO2. ..

Article Title: The GPER is an important factor through which somatic cells regulate oocyte maternal mRNA translation and developmental competence.
Article Snippet: The G protein-coupled estrogen receptor (GPER) plays a crucial role in various biological processes, but its regulation of oocyte meiosis remains unclear.. In this study, we generated a Gper1 knockout in growing oocytes using Zp3-Cre, revealing that GPER is essential for oocyte maturation and embryo development.. RNA-seq analysis indicated that GPER deficiency significantly altered the oocyte transcriptome and disrupted mRNA translation.

Incubation:

Article Title: Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility.
Article Snippet: .. In brief, ITT fragments were mechanically dissected using a disposable scalpel and needles in first enzymatic solution [0.1 % w/v collagenase I-A (Sigma-Aldrich, St. Louis, USA) and 1 % (vol/vol) Pen–Strep in Minimum Essential Medium (MEM)- α (Thermo Fisher Scientific, Waltham, USA)] followed by incubation at 37 ◦C with gentle shaking. .. After a 10-min settling period, the supernatant was collected, washed, and the sediment was subjected to further digestion in a second enzymatic solution [0.1 % w/v collagenase I-A, 0.05 % w/v DNAse I (Sigma-Aldrich, St. Louis, USA), 0.05 % w/v hyaluronidase (Sigma-Aldrich, St. Louis, USA) and 1 % (vol/vol) Pen–Strep in MEM-α], and incubated at 37 ◦C with gentle shaking.

Gentle:

Saline:

Article Title: Enhancing osteogenic differentiation of mesenchymal stem cells seeded on a polycaprolactone/MoS<sub>2</sub> nanofibrous scaffold through electrical stimulation
Article Snippet: Also, 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) (MTT), dimethyl sulfoxide (DMSO) and isopropanol were purchased from Merck (Germany) and used without further purification. .. Fetal bovine serum (FBS), phosphate buffer saline (PBS), penicillin streptomycin solution, minimum essential medium (MEM) α, and trypsin enzyme were acquired from Gibco (Germany). ..

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